Dear Rana, Did your protein come out from the void volume of gel filtration column? In my case, I have expressed a soluble aggregated protein which always like to interact with MBP.
Kind regards, Wenhe > On 27 Mar, 2014, at 6:26 pm, rana ibd <rna19792...@yahoo.com> wrote: > > Dear CCP4 > Does anyone know how to remove the maltose binding protein after cleavage > from the target protein; I have tried gel filtration and ion exchange but > with no luck, my protein is interacting with the MBP even after complete > cleavage. I would be grateful for any help or suggestions > Best Regards > Rana
