Dear Tassos, Do you know by chance whether BLEND is available?
Best wishes, Alex On 27 Mar 2014, at 16:06, Tassos Papageorgiou <tassos.papageorg...@btk.fi> wrote: > Hi, > > You may also try BLEND to choose the optimal data sets before scaling and > merging > > Foadi J, Aller P, Alguel Y, Cameron A, Axford D, Owen RL, Armour W, Waterman > DG, Iwata S & Evans G (2013) Clustering procedures for the optimal selection > of data sets from multiple crystals in macromolecular crystallography. Acta > Crystallogr D Biol Crystallogr 69: 1617–1632 > > Tassos Papageorgiou > > Jarrod Mousa wrote: >> Hi, >> I am trying to solve the structure of a membrane protein. The protein has 12 >> helices and I have a good molecular replacement model that seems to work for >> about half of the structure. I used chainsaw to convert the amino acid >> residues to that of my protein sequence, and the density fits the structure >> well on one side of the protein, but on the other side (about 5 helices), >> there doesn't seem to be any density for the side chains. Has anyone had >> experience with this? The completeness is high ~99% for 3.2 angstroms. The >> data was collected from fairly small crystals ~ 20um. >> Thanks. -- Alex Batyuk The Plueckthun Lab www.bioc.uzh.ch/plueckthun
