Hi Theresa,
Check if this works in your case
http://www.sciencedirect.com/science/article/pii/S0969212608000609

good luck !!


On Sat, Apr 12, 2014 at 4:38 PM, Theresa Hsu <theresah...@live.com> wrote:

> Dear all
>
> Does anyone has experience with Thermofluor assay to find the substrate
> transported/binding by a membrane protein? My protein does not have any
> similar structures and the substrate suggested by sequence analysis is not
> being transported in proteoliposome. I know ITC is good but I am looking
> for a more high-throughput way.
>
> Thank you.
>



-- 
rashmi

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