Attenuate the beam and take many more frames such that you get a full dataset, albeit at slightly lower resolution, before the metals go away. In other words, reduce dose/degPhi.
JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dean Derbyshire Sent: Wednesday, April 30, 2014 6:33 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] metals disapear Hi all, Has anyone experienced catalytic metal ions disappearing during data collection ? If so, is there a way of preventing it? D. Dean Derbyshire Senior Research Scientist [cid:image001.jpg@01CF6450.2D6A0D80] Box 1086 SE-141 22 Huddinge SWEDEN Visit: Lunastigen 7 Direct: +46 8 54683219 www.medivir.com<http://www.medivir.com> -------------------------------------------------------------------------- This transmission is intended for the person to whom or the entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, please be notified that any dissemination, distribution or copying is strictly prohibited. If you have received this transmission in error, please notify us immediately. Thank you for your cooperation.
