Dear All, So there has been quite a bit of advice on minimising radiation damage, and on some of the effects of radiation damage, but unless I have missed it no-one has come up with a clear cut case where radiation damage actually resulted in the (complete ?) loss of a metal ion.
This makes Felix's question all the more relevant: In this particular case, is there any certainty that the metal was there in the first place ? I know, for example, that the catalytic metals are often missing in the binary complexes of polymerases (protein + oligo but no incoming NTP), but this is not due to radiation damage. Equally, in F1-ATPase we sometimes have a nucleotide bound without the coordinative Mg ion. I realise that Dean may not be able to give full details as this may be commercially sensitive. Best wishes, Andrew On 30 Apr 2014, at 11:33, Dean Derbyshire <dean.derbysh...@medivir.com> wrote: > Hi all, > Has anyone experienced catalytic metal ions disappearing during data > collection ? > If so, is there a way of preventing it? > D. > > Dean Derbyshire > Senior Research Scientist > <image001.jpg> > Box 1086 > SE-141 22 Huddinge > SWEDEN > Visit: Lunastigen 7 > Direct: +46 8 54683219 > www.medivir.com > > -------------------------------------------------------------------------- > This transmission is intended for the person to whom or the entity to which > it is addressed and may contain information that is privileged, confidential > and exempt from disclosure under applicable law. If you are not the intended > recipient, please be notified that any dissemination, distribution or copying > is strictly prohibited. If you have received this transmission in error, > please notify us immediately. > Thank you for your cooperation.