Thank you everyone...
On Sun, Aug 17, 2014 at 10:35 PM, Bernhard Rupp (Hofkristallrat a.D.) < hofkristall...@gmail.com> wrote: > Also MATTPROB indicates 3 or 4 molecules/asu as likely solutions. > > http://tinyurl.com/pe2choc > > I do not understand why you propose 16 mol/asu? > > The entire unit cell would likely be only 8 molecules (2 tetramers).... > > BR > > -----Original Message----- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Remy Loris > Sent: Sunday, August 17, 2014 4:45 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] > > A back of the envellope calculation shows that your asymmetric unit > contains most likely 4 molecules with Vm = 2.16 A3/Da, corresponding to 43% > solvent. Searching for 16 molecules is thus nonsense. > > Remy Loris > Vrije Universiteit Brussel > > On 17/08/14 08:54, Avisek Mondal wrote: > > Hello everyone, i am struggling with a problem.. My crystal was > > diffracted at 1.9A in P21 spacegoup with unit cell parameter a=87.7 > > > > b=93.9, c=111.78 ,beta=94.98 which contains 16 molecules per > > assymmetric unit (Molecular weight of the Protein+DNA =56 KDa. > > > > actually it is a complex of 40Kda tetramer and 23bpDNA ) .In solution, > > it shows tetrameric in nature The crystal structure of its > > > > homologous structure has been reported earlier (50%identical in amino > > acid seq.) and it was also a tetramer and its unit cell (also P21) > > > > was approximately 4 times less than mine. It showed 4 molecules per > > assymmetric unit. I didn't get any molecular replacement. All > > > > the programms are takings very long time to do it. Although the single > > crystal is untwinned i think it is a case of pseudosymmetry. > > > > Please help me if you have any good suggestion regarding molecular > > replacement other than experimental phasing. > > > >
