Hi Prashant, I typically stop the centrifuge once in awhile and pipet up/down to prevent the sample from over-concentrating. Depending on how sensitive the sample is, you may want to do this once every 10-60 min.
Hope this helps, Chris On Tue, Aug 19, 2014 at 1:42 PM, Prashant Deshmukh < prashantbiophys...@gmail.com> wrote: > Hi, > i am concentrating my protein using centricon filter, but it is > precipitated soon. Please help me solving this problem. > Thanks. > Prashant Deshmukh > Dept. of Biophysics, > NIMHANS, > Bangalore 560 029, > E-mail:prashantbiophys...@gmail.com > Mob.No.: +919620986525 >
