Fabrice Gorrec, at Cambridge, made the MORPHEUS screen and I really, really like it http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246824/ It's easy to mix yourself and if you make extra of the stocks it is also very easy to mix a follow-up grid screen. The conditions are cryoprotected from the outset (which I very much agree with - if your initial hits from other screens freeze with ice rings then you should attempt to optimize towards cryoconditions rather than cryoprotect crystals after they have grown, in my opinion). I disagree with the choice to include calcium + magnesium since I think you should have screened your protein to find out if it binds any metal ions before you start crystallization screening (since it will also influence your optimal purification protocol). If you have stabilizing salts or other factors you can try setting up the same screen multiple times but with different protein buffer. pH is restricted to 6.5, 7.5, and 8.5 but that's where the majority of hits occur anyway. And the grid optimization goes both higher and lower than those max / min values.
I've had great success with Morpheus. Morten On 17 October 2014 00:15, xaravich ivan <xaravich.i...@gmail.com> wrote: > Hi everyone, > Several of you wanted to know what kind of suggestions I received. > Attached you will find all the replies. > > Thanks > > On Thu, Oct 16, 2014 at 9:57 AM, xaravich ivan <xaravich.i...@gmail.com> > wrote: > >> Dear Xtallographers, >> >> As the budget becomes tighter it is difficult to get hold of all the >> crystal screens one would want to try, to crystallize a protein. >> >> What in your opinion/experience is/are the first few commercial crystal >> screens you would try? ( or what are the ones you routinely start with and >> have got more success with?) >> >> With a very limited budget and without crystal robot access ( which means >> larger volumes of screens/ well) I would like to know your personal >> favorites. >> >> >> Thanks >> Ivan >> > > -- Morten K Grøftehauge, PhD Pohl Group Durham University
