Hi everyone, Thanks for the advice. I had, in fact, already ordered some commercial couplers, but they haven't come in yet, and there was an experiment I wanted to do today.
Here's what I found: 1. The steel ferrules have an orientation, with a "tight" side and a loose side. They can be removed by sliding in one direction, but not the other. Even then, it's pretty difficult. I had to use pliers, and pieces of needle broke off during the process. The tight side of the ferrule then needed to be reamed open slightly with a steel tool before I was able to slide the ferrule onto the other needle. 2. Soldering stainless steel is really a pain. In the end I got a coupler that seems to work well, but it was a pain, and it's a bit charred looking. Thanks again, Tom On Mon, Dec 15, 2014 at 6:01 PM, Aaron Thompson <aaron.a.thomp...@gmail.com> wrote: > I agree with Jim – purchasing the couplers will get you up and running much > quicker. > > TTP also sells nice couplers: > https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050&sessionkey=# > > Aaron > > > > On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp <hofkristall...@gmail.com> > wrote: >> >> Tried the RN kludge.... at least I did not get it to work. You cannot >> tighten the plastic swage lock type sleeves >> tight enough. On operation the pressure drives the PEEK tubing out of the >> compression fit. >> Maybe if you have a jig that holds the 2 syringes in a fixed position so >> they cannot move apart it can work. >> >> Best, BR >> >> -----Original Message----- >> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of >> Daniel Anderson >> Sent: Monday, December 15, 2014 8:48 PM >> To: CCP4BB@JISCMAIL.AC.UK >> Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP >> preparation >> >> Here's my addition to Jim Fairman's reply: >> >> You could use a pair of RN compression fittings (www.hamilton dot com part >> number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field >> of view can have an inside diameter as small as 0.005 inch. >> >> hope that helps, Happy Merry, etc., >> Dan >> >> >> On 12/15/2014 11:09 AM, Thomas Cleveland wrote: >> > Hi all, >> > >> > I'm trying to put together some homemade syringe couplers following >> > the published instructions from the Caffrey group. I'm having a bit >> > of trouble with this part: >> > >> > "The stainless steel ferrule of the second needle is removed and >> > placed on the free end of the coupling needle such that the double >> > thumb nut is held symmetrically between the two steel ferrules" >> > >> > Has anyone done this, and if so, how did you remove the stainless >> > steel ferrule from the second needle in order to place it over the >> > first? The stainless steel ferrule appears to be firmly attached and >> > I'm having trouble removing it. >> > >> > Thanks, >> > Thomas Cleveland
