Mohamed, You always list the sequence of what's actually in the crystal, e.g. 1-105. (Not: what's in the model or what the sequence of the full length protein is). Make sure that if there's any lingering residues from any affinity/purification tags they get included in the sequence too.
Phil Jeffrey Princeton ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Mohamed Noor [mohamed.n...@staffmail.ul.ie] Sent: Tuesday, December 30, 2014 3:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] PDB deposition - sequence file Dear all The protein that was crystallized is only the first 105 residues of a 230-residue protein. In the structure, I can see density for residues 6-72. For deposition, should the whole native/biological sequence be deposited? Thanks. Mohamed
