I would disagree. You list as sequence what you cloned and expressed. If you are missing parts then you have to describe why e.g. Proteolysis or disordered etc. Best to actually do a mass spec analysis on those crystals since a significant portion is missing.
Jūrgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street<x-apple-data-detectors://4>, W8708 Baltimore, MD 21205<x-apple-data-detectors://5/0> Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://lupo.jhsph.edu<http://lupo.jhsph.edu/> On Dec 30, 2014, at 15:50, Jeffrey, Philip D. <pjeff...@princeton.edu<mailto:pjeff...@princeton.edu>> wrote: Mohamed, You always list the sequence of what's actually in the crystal, e.g. 1-105. (Not: what's in the model or what the sequence of the full length protein is). Make sure that if there's any lingering residues from any affinity/purification tags they get included in the sequence too. Phil Jeffrey Princeton ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] on behalf of Mohamed Noor [mohamed.n...@staffmail.ul.ie<mailto:mohamed.n...@staffmail.ul.ie>] Sent: Tuesday, December 30, 2014 3:26 PM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] PDB deposition - sequence file Dear all The protein that was crystallized is only the first 105 residues of a 230-residue protein. In the structure, I can see density for residues 6-72. For deposition, should the whole native/biological sequence be deposited? Thanks. Mohamed
