Try adding b-ME/DTT/TCEP in your crystallization conditions, if its not there already in your protein buffer.
On Mon, Jan 13, 2014 at 2:02 PM, Debasish Chattopadhyay <debas...@uab.edu> wrote: > We crystallized a protein at 4 and 22 deg C in different conditions: > > > > from ammonium sulfate in acetate buffer pH 5 > > and > > PEG4000 in Hepes buffer at pH 7.5 > > > > In both cases the drops have a slimy skin (almost feels like DNA). We > therefore think that the skin is generated from the protein. > > > > I am sure some of you have had similar experiences. I would like your > suggestions about how to avoid the skin. Please note that we are *not* > asking for suggestions on how to handle the skin (such as using various > tools) *we are only interested in knowing if there is a way to prevent > the formation of the skin*. > > > > Thank you so much > > > > Debasish > > > > Ph: (205)934-0124; Fax: (205)934-0480 > > > -- Gyanendra Kumar, PhD St. Jude Children's Research Hospital, Department of Structural Biology, 262, Danny Thomas Place, MS-311 Memphis, TN 38105 Phone: 901-595-3839 Cell: 631-875-9189 -------------------------------------------------------
