Sudipta, the link for the cyanin dyes are at the following link http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-stains.html
Sorry for the wrong link P On Fri, Feb 13, 2015 at 1:50 PM, Sudipta Bhattacharyya < sudiptabhattacharyya.iit...@gmail.com> wrote: > Dear Community, > > Sorry for this off topic question. I am dealing with a non specific DNA > binding protein. In a non radio-labelled EMSA DNA:protein titration > experiment when I EtBr stained the 5% native acrylamide gel, I could see > the DNA control (101 bps) but as the amount of my protein increases I saw > the free DNA band getting thinner and thinner but I could not see any band > shift and probably all the complex stuck at wells. But, even if I assume > the whole DNA length is occupied by my protein (I determined the ratio to > be 1:15 ish) the molecular weight should not reach the molecular weight of > the highest DNA marker band (20kbps = 13,200 kDa). Then why I could not see > a retarded band in the gel? Or is it forming a EtBr resistant nucleoprotein > filament? Could you guys please give me an idea/suggestions? > > Many thanks in advance...!!! > > My best regards, > Sudipta. > -- P
