Dear all,
I am trying to express my protein in minimal medium M9 (trace elements + biotin and thiamine, according to Doublie protocol) but, after O.N. incubation there wasn't growing.I tried to change vitamins concentration and in the end just had good results when I put 0,1% yeast extract. So, for this I would like some help... there is in yeast extract large quantities of methionine? Because, if yes it can put down my essay with semet. P.S 1. I put the bacterials first in L.B, then in solid minimal medium M9 where they growed normally and when i inoculate in the same minimal medium M9 composition but liquid form, there wasn't growing. 2. My e.coli strain is roseta (de3) Thanks in advance Best Regards, Giulliana -- Giulliana Rangel M.S. Biotechnology Program - UNIFESP Structural Biology Laboratory Phone: +55 (12) 3309-9698 330 Talim St ZIP CODE 12231-280 São José dos Campos - SP/ BR