I know there was jut recently a discussion about cryoconditions for crystals, but I am still hoping for some new ideas for my crystals that grow from HEPES buffer pH 7.3, 0.2 M NaCl by slowly lowering the temperature from 20 to 4 degrees.
These crystals are easy to grow but extremely sensitive to temperature change, and any of the usual cryo reagents tested so far simply dissolve the crystals. I am n ot sure how I could counteract the solubilizing effect of glycerol or ethylen glycol, since there is no precipitant concentration that I could increase to stabilize the crystals. Paratone didn't work either so far. Any other ideas for these low salt crystals? Ursula -- Ursula Schulze-Gahmen, Ph.D. Project Scientist UC Berkeley, QB3 360 Stanley Hall #3220 Berkeley, CA 94720-3220 (510) 643 9491
