Thanks for all the good suggestions. I guess I really should try the non-frozen data collection first.
Ursula On Mon, Mar 2, 2015 at 11:07 AM, Keller, Jacob <kell...@janelia.hhmi.org> wrote: > What about 4deg data collection? > > > > What about glutaraldehyde crosslinking? > > > > JPK > > > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Ursula > Schulze-Gahmen > *Sent:* Monday, March 02, 2015 1:49 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] cryo protection for low salt crystallization at 4 > degrees > > > > I know there was jut recently a discussion about cryoconditions for > crystals, but I am still hoping for some new ideas for my crystals that > grow from HEPES buffer pH 7.3, 0.2 M NaCl by slowly lowering the > temperature from 20 to 4 degrees. > > These crystals are easy to grow but extremely sensitive to temperature > change, and any of the usual cryo reagents tested so far simply dissolve > the crystals. I am n ot sure how I could counteract the solubilizing effect > of glycerol or ethylen glycol, since there is no precipitant concentration > that I could increase to stabilize the crystals. Paratone didn't work > either so far. > > Any other ideas for these low salt crystals? > > Ursula > > > -- > > Ursula Schulze-Gahmen, Ph.D. > Project Scientist > UC Berkeley, QB3 > 360 Stanley Hall #3220 > Berkeley, CA 94720-3220 > (510) 643 9491 > -- Ursula Schulze-Gahmen, Ph.D. Project Scientist UC Berkeley, QB3 360 Stanley Hall #3220 Berkeley, CA 94720-3220 (510) 643 9491
