Just to clarify, I think what Kay meant with "strategy" is that you don't just shoot at the crystal and collect. You should figure out what is the optimum start and end point of your data collection. Best to be cautious and not immediately go for highest resolution and not fry your crystal. A 4 A complete anomalous data set is better than a partial 3.2A one. J?rgen
...................... J?rgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street<x-apple-data-detectors://4>, W8708 Baltimore, MD 21205<x-apple-data-detectors://5/0> Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://lupo.jhsph.edu<http://lupo.jhsph.edu/> On Apr 17, 2015, at 06:37, Kay Diederichs <kay.diederi...@uni-konstanz.de<mailto:kay.diederi...@uni-konstanz.de>> wrote: Hi, I'd say using a Pilatus detector in fine-slicing mode and lowdose/high multiplicity will give you better chances to solve the structure. The right strategy makes a difference ... Best, Kay
