I’m sure James Holton has an option for that :-) By the way, zero photon data sets exist and have been published before (some of them had to be retracted though).
Jürgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://lupo.jhsph.edu On Apr 17, 2015, at 11:00 AM, Keller, Jacob <kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>> wrote: Finally, there is simply no downside in collecting more degrees with proportionally lower dose on the Pilatus. Merging the data recovers the _same_ signal. It has only advantages - so many that I won't write them up here with 1 finger on my tablet. ...Up to the point at which one can no longer index/refine the frames reliably. And it would be interesting to try to figure out what that point is, or how to push it to even fewer photons. JPK With a CCD it's a different story. Best, Kay Am 17. April 2015 15:49:21 MESZ, schrieb Jurgen Bosch <jbos...@jhu.edu<mailto:jbos...@jhu.edu>>: I would disagree. My philosophy is: assume this is your only diffracting crystal, maximize the outcome by investing some thoughts into it before being sorry. Therefore, run strategy and optimize for anomalous pairs being collected as close in time as possible. If you have the luxury of having multiple crystals you know diffract, then it;s a different story. Regarding the 1degree option, I think that dates back to the dinosaurs of crystallography, when only non-decimal numbers were an option to be entered in the CLI, yes there was no GUI before :-) Also the goniometers are much more accurate these days. More seriously, I think this had something to do perhaps with the cost of storage, remember 50 MB was a lot of space 20 years ago. Your average Pilatus data set today comes at 3-5 GB, considering a 6TB drive costs about 250$ today that’s nothing. Or reading the files from a DAT4 drive took ages, so you really didn’t want to collect fine sliced data. Jürgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://lupo.jhsph.edu On Apr 17, 2015, at 9:25 AM, Kay Diederichs <kay.diederi...@uni-konstanz.de<mailto:kay.diederi...@uni-konstanz.de>> wrote: Hi Jürgen, sorry - that's what I get when mailing while boarding ... No, I'd just collect 360 degrees, and if the crystal is still ok, another 360, ... This way one - obtains high completeness and multiplicity - can discard frames with "too much" radiation damage - does not have to worry about the starting point of data collection. To make the most of the second 360°, you should change some parameter: wavelength, rotation axis (requires a BL with kappa or Prigo), or at least distance (by few percent). When I read that 1° frames are collected, I just wonder why? Because it used to be done like that in the good old times? HTH, Kay On Fri, 17 Apr 2015 11:55:42 +0000, Jurgen Bosch <jbos...@jhu.edu<mailto:jbos...@jhu.edu>> wrote: Just to clarify, I think what Kay meant with "strategy" is that you don't just shoot at the crystal and collect. You should figure out what is the optimum start and end point of your data collection. Best to be cautious and not immediately go for highest resolution and not fry your crystal. A 4 A complete anomalous data set is better than a partial 3.2A one. J?rgen ...................... J?rgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street<x-apple-data-detectors://4>, W8708 Baltimore, MD 21205<x-apple-data-detectors://5/0> Office: +1-410-614-4742<tel:%2B1-410-614-4742> Lab: +1-410-614-4894<tel:%2B1-410-614-4894> Fax: +1-410-955-2926<tel:%2B1-410-955-2926> http://lupo.jhsph.edu<http://lupo.jhsph.edu/> On Apr 17, 2015, at 06:37, Kay Diederichs <kay.diederi...@uni-konstanz.de<mailto:kay.diederi...@uni-konstanz.de>> wrote: Hi, I'd say using a Pilatus detector in fine-slicing mode and lowdose/high multiplicity will give you better chances to solve the structure. The right strategy makes a difference ... Best, Kay -- Diese Nachricht wurde von meinem Android-Mobiltelefon mit K-9 Mail gesendet.
