Hey Veera, you have missed to assign all labels which were required as input parameters. DM is trying to use your HLA labels for density modification. Either you assign them if present or you choose to omit these and just use FHI and FOM labels. This can be done in the header of the DM GUI.
Best Schara > Am 27.04.2015 um 19:38 schrieb Sekharreddy M <vijayasekk...@gmail.com>: > > Hi, > I was trying to do density modification using DM,CPP4 suit, but not > able to run as I am getting an error msg: > > FP = DELFWT SIGFP = SIGF_l5l6_009_p4212 PHIO = PHIC FOMO = Unassigned HLA = > Unassigned HLB = Unassigned HLC = Unassi > Data line--- LABOUT > <x-msg://13/programs/x86_64-linux/ccp4/6.4.0/ccp4-6.4.0/html/dm.html#labout>FDM=FDM > PHIDM=PHIDM FOMDM=FOMDM HLADM=HLADM HLBDM=HLBDM HLCDM=HLCDM HLDDM=HLDDM > > dm: (RCARDS) SOLC: content missing or out-of-range > Times: User: 0.0s System: 0.0s Elapsed: 0:00 > > I am just familiarizing with the crystallization structure solving software > suits, can any body please help me in finding out the reason for the DM run > failure. > > > The inputs I gave are just mtz file after refinement and solvent content(in > my case 69%) and the parameters selected for the DM run are: > > 1. Density modification modes : selected only:solvent and histogram > > 2.Selected phase combinations are perturbation and all reflection > > 3.Create map file in CCP4 FORMAT > > 4.Phase extension cycles:10 > > 5.HLA, HLB,HLC , HLD and weight are not selected (I don't know where do I > find the information about these to feed ) > > 6. FP: DELFWT, PHIO: PHIC, WEIGHT :Unassigned, SIGFP: Automatically selected > after I gave my mtz file > > Thank you > Veera
