Dear all Sorry for going a bit off-topic in this thread. May I seek your advice as on whether you have experienced that crystals being obtained from the same droplet, looking alike under microscope (rod shape) and in fact growing possibly from a same nuclei, give two space groups after indexing?
I recently obtain crystals for a protein (co-crystallized with a nucleic acid ligand) and collected two datasets from synchrotron. Although these two crystals are from the same drop, the SG and unit cell dimensions are very different: Xtal1: C121 (156 60 105 90 111 90) (L-test, Pointless shows that there is no twinning), ~2.5 Angstrom Xtal2: P1 (53 60 79 106 105 98), ~3 Angstorm Would it be possible that the ligand changes the SG of the crystal so that only one of the forms contains the ligand? Any advice is appreciated and thanks a lot in advance for your input. Regards Sam Tang Biochemistry Programme, School of Life Sciences, CUHK
