Dear all Thanks a lot for the numerous input which is highly appreciated.
I should provide some updates as to what I have done on these two crystals / datasets. I processed both datasets with HKL2000 as well as imosflm. Both softwares give (essentially) the same unit cells as in the first email. The C2 cell doesn't look like a transformation of the P1 cell. However Phaser (and in fact Xtriage) identified that there is translational pseudosymmetry in the C2 dataset but not the P1 dataset. We are lucky enough to obtain MR solutions for the dataset but we remain a bit cautious about the C2 we had. Currently we are looking at Zanuda to see if this is what it should be. Thanks again! Kind regards Sam On 30 October 2016 at 14:54, Kevin Jude <kevinmj...@gmail.com> wrote: > 3ICE had two space groups in the same /crystal/ - P6 at one end, P1 with > pseudo-6-fold NCS at the other. In the P6 case, the RNA ligand (in the > center of a hexameric protein) was rotationally averaged, but in the P1 > case it could be resolved. > > Best wishes > Kevin > > On Fri, Oct 28, 2016 at 6:13 AM, Sam Tang <samtys0...@gmail.com> wrote: > >> Dear all >> >> Sorry for going a bit off-topic in this thread. >> May I seek your advice as on whether you have experienced that crystals >> being obtained from the same droplet, looking alike under microscope (rod >> shape) and in fact growing possibly from a same nuclei, give two space >> groups after indexing? >> >> I recently obtain crystals for a protein (co-crystallized with a nucleic >> acid ligand) and collected two datasets from synchrotron. Although these >> two crystals are from the same drop, the SG and unit cell dimensions are >> very different: >> >> Xtal1: C121 (156 60 105 90 111 90) (L-test, Pointless shows that there is >> no twinning), ~2.5 Angstrom >> Xtal2: P1 (53 60 79 106 105 98), ~3 Angstorm >> >> Would it be possible that the ligand changes the SG of the crystal so >> that only one of the forms contains the ligand? >> >> Any advice is appreciated and thanks a lot in advance for your input. >> >> Regards >> >> Sam Tang >> Biochemistry Programme, School of Life Sciences, CUHK >> >> >
