Dear All, Sorry for the little bit off topic. Is there a possibility for covalent bond formation between beta phosphate of ADP and acetate molecule both are coordinated by divalent metal ions? I am working on a Kinase structure which was crystallized with ADP and in presence of 1M sodium acetate. We observed additional density around ADP that fits perfectly like a gamma phosphate , mimicking like ATP bound state, surrounded and coordinated by two metal ions(resolution is 1.4A). There is a change in space group (from I212121 to P212121 ) and further important conformation changes are observed around ATP binding pocket and distant region. This is the only xtal we obtained in this space group, and all other xtals(measured 10 xtals) from the same plate belong to I212121.
Thanks your help and time! Saravanan ----- Original Message ----- From: "CCP4BB automatic digest system" <lists...@jiscmail.ac.uk> To: CCP4BB@JISCMAIL.AC.UK Sent: Sunday, 15 January, 2017 01:01:37 Subject: CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) There are 2 messages totaling 45 lines in this issue. Topics of the day: 1. Phenix (2) ---------------------------------------------------------------------- Date: Sat, 14 Jan 2017 20:58:09 +0000 From: D Bonsor <dbon...@ihv.umaryland.edu> Subject: Phenix Is the phenix website down? Anyone know when it will be back up? ------------------------------ Date: Sat, 14 Jan 2017 14:45:06 -0800 From: Pavel Afonine <pafon...@gmail.com> Subject: Re: Phenix See notice on Phenix mailing list that answers your question. On Sat, Jan 14, 2017 at 12:58 PM, D Bonsor <dbon...@ihv.umaryland.edu> wrote: > Is the phenix website down? Anyone know when it will be back up? > ------------------------------ End of CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) ************************************************************
