Dear All, Sorry for the off-topic question, I'd like to do Biacore SPR assay with N-terminal biotinylated peptide as ligand (to Biacore SA chip) and my protein as analyte. I have a question of how to determine the concentration of biotinylated peptide （synthetic peptide), if the peptide has no Tyr and no Trp residue, I guess amino acid analysis may not work because the N-terminal of the peptide is biotinylated.
I'd appreciate if anyone share his/her experience on this.
