Assuming the protein is in solution, drop cast 2ul of solution onto a glass surface and spread evenly with a sharp needle. Air dry in dust free chamber and blow the surface with gentle stream of nitrogen gas. Proteins stick to surface quite well to be analyzed for various microscopy (AFM, SEM etc.). If the purpose is not to do any surface probe microscopy, coating the glass surface with Sigmacoat helps in better binding. Poly-lysine coated glass cover slips can be alternative but mostly not preferred for small proteins or peptides.
Hope that helps. Best!! Debasish CSIR- Senior Research Fellow (PhD Scholar) C/o: Dr. Akash Ranjan Computational and Functional Genomics Group Centre for DNA Fingerprinting and Diagnostics Hyderabad, INDIA Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com Telephone: 0091-9088334375 (M), 0091-40-24749396 (Lab) Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html ----- Original Message ----- From: Jacob Keller <kell...@janelia.hhmi.org> To: CCP4BB@JISCMAIL.AC.UK Sent: Wed, 05 Apr 2017 08:49:40 +0530 (IST) Subject: [ccp4bb] Off-topic: Attach His-tagged Protein to Coverslip Does anyone have a simple way to attach purified his-tagged protein solidly to a coverslip? Thanks, Jacob Keller ******************************************* Jacob Pearson Keller, PhD Research Scientist HHMI Janelia Research Campus / Looger lab Phone: (571)209-4000 x3159 Email: kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org> *******************************************
