One possibility: surface-immobilised forms of the peptide HGGHHG bind strongly to His tags (coordinating around zinc rather than nickel). See https://www.ncbi.nlm.nih.gov/m/pubmed/15050643/. If you want true permanence, you could apply the trick used on BiaCore chips. They use a NTA-functional carboxymethyldextran surface. His-tagged proteins are captured as usual, then rinsed and made permanent by incubating with some water-soluble carbodiimide to link the carboxymethyl groups to surface lysines - the advantage being that all the proteins are in essentially the same orientation. Not sure if anyone sells coverslip equivalents.
Best regards, Tristan Tristan Croll Research Fellow Cambridge Institute for Medical Research University of Cambridge CB2 0XY > On 5 Apr 2017, at 04:19, Keller, Jacob <kell...@janelia.hhmi.org> wrote: > > Does anyone have a simple way to attach purified his-tagged protein solidly > to a coverslip? > > Thanks, > > Jacob Keller > > ******************************************* > Jacob Pearson Keller, PhD > Research Scientist > HHMI Janelia Research Campus / Looger lab > Phone: (571)209-4000 x3159 > Email: kell...@janelia.hhmi.org > ******************************************* >
