Hi all,

I am solving structure of one of the acyltransferse protein. We have
collected data at 2.16A resolution. Currently the Rfree is 0.2508 and Rwork
is 0.2042.

*My query is regarding NCS difference.* Under validation tool of coot while
looking for NCS differene, i can find some residues with red bar. *Can some
one suggest me how may i minimize it if i am expected to do it?*


-- 
Vipul Panchal
Senior Research Fellow,
Respiratory disease and biology,
CSIR-IGIB
(M)-9540113372

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