Hi everyone, We have a structure that phaser found a solution for in P1, even though there is a large off-origin Patterson peak indicating pseudo NCS. However, the 2mFo-Fc map needs to be contoured up to >3 sigma to be reasonable, going lower makes it look like what I normally see at <1 sigma. If you look at the Fo-Fc map, there are large areas of positive and negative density. The 2mFo-Fc map looks very convincing, you can see the tyrosines, phenyl rings, etc...Also phaser gave LLG of >400 and a TFZ of ~14. Rfree is ~0.45. Does anyone know what's going on?
Thanks, Roger -- Roger Shek Stony Brook University Graduate Student in Biochemistry and Structural Biology (PhD) Stony Brook Integrative Structural Biology Organization <https://sbintstrbio.wordpress.com/> Cell: (808) 386-3879
