I searched for 4 copies, but Phaser only found 3. It's possible there is an additional copy of the molecule in the ASU
On Wed, Apr 26, 2017 at 2:40 AM, Randy Read <rj...@cam.ac.uk> wrote: > Dear Roger, > > You didn’t mention this, but did you search for 2 copies (or an even > number of copies) and, if so, did Phaser take account of the translational > NCS in the calculation? > > Best wishes, > > Randy Read > > ----- > Randy J. Read > Department of Haematology, University of Cambridge > Cambridge Institute for Medical Research Tel: +44 1223 336500 > Wellcome Trust/MRC Building Fax: +44 1223 336827 > Hills Road > E-mail: rj...@cam.ac.uk > Cambridge CB2 0XY, U.K. > www-structmed.cimr.cam.ac.uk > > > On 26 Apr 2017, at 02:23, Roger Shek <roger.s...@stonybrook.edu> wrote: > > > > Hi everyone, > > > > We have a structure that phaser found a solution for in P1, even though > there is a large off-origin Patterson peak indicating pseudo NCS. However, > the 2mFo-Fc map needs to be contoured up to >3 sigma to be reasonable, > going lower makes it look like what I normally see at <1 sigma. If you look > at the Fo-Fc map, there are large areas of positive and negative density. > The 2mFo-Fc map looks very convincing, you can see the tyrosines, phenyl > rings, etc...Also phaser gave LLG of >400 and a TFZ of ~14. Rfree is ~0.45. > Does anyone know what's going on? > > > > Thanks, > > Roger > > > > -- > > Roger Shek > > > > Stony Brook University > > Graduate Student in Biochemistry and Structural Biology (PhD) > > Stony Brook Integrative Structural Biology Organization > > Cell: (808) 386-3879 > > -- Roger Shek Stony Brook University Graduate Student in Biochemistry and Structural Biology (PhD) Stony Brook Integrative Structural Biology Organization <https://sbintstrbio.wordpress.com/> Cell: (808) 386-3879 -- Roger Shek Stony Brook University Graduate Student in Biochemistry and Structural Biology (PhD) Stony Brook Integrative Structural Biology Organization <https://sbintstrbio.wordpress.com/> Cell: (808) 386-3879
