Hi Vipul, The first thing to do is to check whether the fit of the offending residues in the electron density maps is correct. If it is not, you have to do rebuilding. If the fit is correct, I would leave them as they are. Assuming the distribution of bond lengths and angles has a bell-shape, there will always be some residues at the extreme ends.
Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Vipul Panchal Gesendet: Mittwoch, 17. Mai 2017 14:34 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Bond length and angle outlier fixing HI all. I am solving protein structure with 2.16A resolution. There are two chain in an asymmetric unit. While submitted to PDB validation server, i could see few ligand bond -length and -angle outlier. Coot doesn't have any module that can help me with these as per my best understanding. Kindly find the image of relevant details attached herewith. Can somebody suggest me how to fix them? Sincerely, -- Vipul Panchal Senior Research Fellow, Respiratory disease and biology, CSIR-IGIB (M)-9540113372
