Dear ALL, I got two kinds of crystals of different proteins ,but there are many similarities.
The shape of the crystals are similar, the cell parameters are also similar : protein A , 136.12 94.398 89.476 90 125.479 90 , Space group C 1 2 1 and protein B , 136.14 94.369 89.115 90 125.495 90 , Space group C 1 2 1. protein A has a NMR structure ,but Rfree always high above 50% after molecular replacement , protein B’s Rfree is also above 50% . So I am wonder if these crystals are the result of debris of proteins , because the growth of the crystals needs more than half a year . I am sure the two proteins are different and crystals respectively come from different proteins Any insights will be really appreciated. Thanks Dong Xiao