Yes, the hinge region between Fab and Fc is highly flexible. If you look at IgG by EM you will see many different conformations. Best to use Fab or scFv.
Jarrod Mousa On Thu, Jun 22, 2017 at 1:39 PM, Cheng Zhang <chengzh1...@gmail.com> wrote: > Hi all, > > I have a naive question about antibodies. Many people used Fab fragments > in crystallization. I am wondering if it is possible to use the whole IgG > molecule with Fc fragment as well. Or it would be too flexible and bad for > crystallization? > > Thanks, > > Cheng > > > -- > --------------------- > Cheng Zhang >
