Hi Chenjun Tang, From the images you sent, it looks like your crystal suffers from lattice translocation disorder. See e.g. http://onlinelibrary.wiley.com/doi/10.1107/S0907444909025153/epdf
Calculating a native Patterson and looking for strange peaks may give some hints what is going on. Depending on the nature of the disorder, you may or may not correct for it. Best, Herman -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Tang Chenjun Gesendet: Donnerstag, 13. Juli 2017 14:21 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] weird diffraction pattern Hi David, Thanks for your comments. Although the spots become streaky in certain directions, I have processed the data in HKL3000 and imosflm, which suggested the C2221 space group (66.59, 246.95 and 210.17). The Rmerge(0.14), completeness(94.8%), redundancy(4.6) are OK. When I tried to run Balbes with the solved native structure, the molecular replacement solution was poor. So I ran Balbes with the split domains of the native structure. Although the solutions were also poor, I found the MR score of one solution above 35. On the basis of this solution, I tried to run Buccaneer and the Rfree could be 0.46. Unfortunately, there are four molecules in the asymmetric unit and it is to hard for me to reduce the Rfree further. All best, Chenjun Tang
