Thanks to all for replying.
If my solution is correct, why R/Rfree not going down from 21/25? I have tried most of the options like Tls, ncs refinement in refmac5 but these options are not helping me, might be I am not expert with using these options. Could someone guide me how to use these options correctly during refinement? On 15-Sep-2017 12:36 PM, "Eleanor Dodson" <eleanor.dod...@york.ac.uk> wrote: > PAK=0 is GOOD - i.e. there are no clashes between symmetry equivalent > molecules. > So I think you have solved your structure. > Eleanor > > On 15 September 2017 at 07:20, rohit kumar <rohit...@gmail.com> wrote: > >> Dear all, >> >> During MR using PHASER with a 1.8 A data, I found that the PAK=0 but all >> the other values are looking fine. >> >> When I run REFMAC5 the R/Rfree values are 25/30 (without water) and 21/26 >> (after adding water). >> >> Just want to know whether my solution is correct or not. if not please >> let me know what strategies I can use to get correct solution. >> As I have tried all the combination in PHASER. >> >> Here I am attaching the result of PHASER . >> >> >> >> [image: Inline image 3] >> >> >> Thank you in advance >> >> -- >> WITH REGARDS >> Dr. Rohit Kumar Singh >> >> >
