Yuvraj This is a tough call. may be you need to look at this database:
http://web.archive.org/web/20111011202903/http://idb.exst.jaxa.jp/db_data/protein/search-e.php Importantly, when you click on "ex" for the tab, "cryoprotectant", a window will pop up and you can read different kinds of cryoprotectants that have been successfully attempted in the past. Off-course, this also depends on type of precipitant that you used for crystallization. Apparently, this seems as glycerol worked better in your case, may be because you have salt-based precipitant. Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Thu, Dec 28, 2017 at 8:28 AM, YUVARAJ I <yuvee...@gmail.com> wrote: > Respected All, > I have crystallized a protein. It is forming big crystals, I have checked > in the home source, > Without cryoprotectant - No diffraction (blank) > with Ethylene glycol- No diffraction (blank) > with PEG 3350 - 7A diffraction and No ice rings > with Glycerol - 3.5A diffraction with ice rings > > I have tried soaking with glycerol different concentrations 10-30% > glycerol from 30 secs to 5 min. > No improvement in the diffraction quality. > > How to improve the diffraction quality of the crystals and avoid the big > ice ring. > > I have attached the diffraction image with this mail. > > Thanks in advance for your valuable suggestions > > Regards > Yuvaraj > > > -- > > > > > > > >
