Hi Hussain,

Crystallization condition is 200 mM CaCl2 and 20% PEG 3350.

I some what realized that it could be Ca++ with coordinated waters. But I
really wanted to have
second opinion from our group.

Thank you every one.

-Rajesh





On Tue, Mar 6, 2018 at 5:37 PM, Hussain Gps <hussain...@gmail.com> wrote:

> Hi Rajesh,
>
> Can you please let everyone know the buffer/reagents involved throughout
> the process.
>
> Also, how strong is the density? In other words, until what contour level
> you see it in the Fo-Fc map?
>
> Anyways, looking at the shape, it looks well coordinated - could be
> cacodylate? Or water, am not sure.
>
> Cheers,
> Hussain
>
> > On 7 Mar 2018, at 9:19 am, Rajesh Kumar <rajesh.p...@gmail.com> wrote:
> >
> > Dear All,
> >
> > Have you had experience with this kind of density? I am wandering what
> this could be?
> >
> > Thank you very much for the help.
> >
> > -Rajesh
> >
> >
> > <Screen Shot 2018-03-06 at 5.15.20 PM.png>
> >
>

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