Dear Liuqing, It could be good to dissolve the crystals to check if the crystallised protein is still intact. If it is truncated then make a new construct based on truncation. It could improve the crystal diffraction as it was the case for me.
Best wishes, Mohinder ---------------------------------------------- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Lessing ---------------------------------------------- > On 4 Jun 2018, at 11:57, Liuqing Chen <519198...@163.com> wrote: > > Hello everyone! > > I get a crystal several months ago, but the crystals diffraction very low > resolution (around 8A) or no diffraction. > My sample buffer is 20mm Hepes ph7.0, 50mm NaCl, my protein pI is 5. > the codition grow crytal is : 30% PEG400, 100mm hepes 7.5, 200mm MgCl2. > > I also tried additive screen, all the crystals appear the same apparence, > even i seeding optimization, have no improve. > the attach is my crystals. > > what should i do next? > > thanks in advance. > sincerely > Liuqing chen > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <20180307_103s_egalc_wizard3_27_1.tif> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
