Just a quick note- even if the crystals appear the same (i.e. the same morphology in a light microscope), that doesn't necessarily mean that they diffract the same. Did you try putting some of these optimised crystals into an x-ray beam? Or as previously suggested, try them at room temperature? Best of luck, tom
Tom Peat Proteins Group Biomedical Program, CSIRO 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 tom.p...@csiro.au ________________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Liuqing Chen <519198...@163.com> Sent: Monday, June 4, 2018 8:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] suggestion of crystallization optimization Hello everyone! I get a crystal several months ago, but the crystals diffraction very low resolution (around 8A) or no diffraction. My sample buffer is 20mm Hepes ph7.0, 50mm NaCl, my protein pI is 5. the codition grow crytal is : 30% PEG400, 100mm hepes 7.5, 200mm MgCl2. I also tried additive screen, all the crystals appear the same apparence, even i seeding optimization, have no improve. the attach is my crystals. what should i do next? thanks in advance. sincerely Liuqing chen ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
