Hello Jiri, I wouldn't choose neither of them, but Co(II). NMR spectroscopists have been using Co(II) to probe Zn(II) sites in proteins for ages... Check out Bertini&Luchinat (*DOI: *10.1021/ar00092a002 <https://pubs.acs.org/doi/abs/10.1021/ar00092a002>), or Vallee&Auld (*DOI: * 10.1021/bi00476a001 <https://pubs.acs.org/doi/abs/10.1021/bi00476a001>). There are many examples of Zn(II) enzymes retaining their activity as Co(II) surrogates, for instance metallo-beta-lactamases (*DOI: * 10.1021/bi100894r <https://pubs.acs.org/doi/full/10.1021/bi100894r>). Best wishes, Javier
On Sun, Jul 29, 2018 at 6:13 PM, chemocev marker <jirivit...@gmail.com> wrote: > Dear All > > I have a questions about the Zn+2 proteases (Thermolysin based Zn+2 site) > > As Zinc is trouble metal when it comes to test the activity. > > Which is better substitute for it. > > Is MgCl2 or MncCl2 > > Why one is preferred over the other. > > best > > Jiri > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Dr. Javier M. González Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET) Universidad Nacional de Santiago del Estero (UNSE) RN9, Km 1125. Villa El Zanjón. (G4206XCP) Santiago del Estero. Argentina Tel: +54-(0385)-4238352 Email <bio...@gmail.com> LinkedIn <https://www.linkedin.com/in/javier-m-gonzalez-inbionatec> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
