From personal experience, many of them really do need Zn++ and some of them can get by on Cd++ :)
However, Zn++ can often be used in the micromolar range (so essentially quasi-stoichiometric with the enzyme) so it's not as much of a PITA as when you have millimolar quantities. HTH! Artem - Cosmic Cats approve of this message On Sun, Jul 29, 2018 at 5:13 PM, chemocev marker <jirivit...@gmail.com> wrote: > Dear All > > I have a questions about the Zn+2 proteases (Thermolysin based Zn+2 site) > > As Zinc is trouble metal when it comes to test the activity. > > Which is better substitute for it. > > Is MgCl2 or MncCl2 > > Why one is preferred over the other. > > best > > Jiri > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
