Sam Would this unit cell index some of the spots? a = 7.00 ± 0.04 A, b = 9.96 ± 0.05 A, c = 6.29 ± 0.04 A. Colin
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Sam Tang Sent: 09 October 2018 12:13 To: ccp4bb <ccp4bb@jiscmail.ac.uk> Subject: [ccp4bb] Weird diffraction pattern Dear all Hello. We recently shot a crystal (a protein with small molecule as ligand) at a synchrotron source and see a weird pattern. (https://drive.google.com/file/d/11bEtTJzKaAB5ZybezgN1cqBrckSRg2OV/view?usp=sharing) Crystal was grown in Citric acid and ammonium sulfate, cryoprotected with glycerol. At first we thought it was a protein crystal contaminated with salt but on second thought, the lowest resolution spot was at around 7 A, which doesn't make sense for a protein. So we would like to solicit your experience and perhaps someone may have encountered similar pattern before? Many thanks. Kind regards Sam ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1