To me, it looks like some intergrown salt crystal. HS Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Sam Tang Gesendet: Dienstag, 9. Oktober 2018 13:13 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] Weird diffraction pattern
Dear all Hello. We recently shot a crystal (a protein with small molecule as ligand) at a synchrotron source and see a weird pattern. (https://drive.google.com/file/d/11bEtTJzKaAB5ZybezgN1cqBrckSRg2OV/view?usp=sharing<https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_file_d_11bEtTJzKaAB5ZybezgN1cqBrckSRg2OV_view-3Fusp-3Dsharing&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=ExvzTpwCzYQcP3Idr9vHnPcGFh0UXDAKsEB7jClqsF0&s=4dUd1KLQYV5G8_jUYdS8kORXsEkS-0kwKqiGRj5dvDg&e=>) Crystal was grown in Citric acid and ammonium sulfate, cryoprotected with glycerol. At first we thought it was a protein crystal contaminated with salt but on second thought, the lowest resolution spot was at around 7 A, which doesn't make sense for a protein. So we would like to solicit your experience and perhaps someone may have encountered similar pattern before? Many thanks. Kind regards Sam ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=ExvzTpwCzYQcP3Idr9vHnPcGFh0UXDAKsEB7jClqsF0&s=Ud5SL2yONTX62SIhpLS7sPJxpTJogstplKUe7cQgN_g&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
