For the last 150,000 years we've been using the Hampton THICK siliconized coverslips - I'm told they're one of the many crystallization tricks/gizmos that Alan D'Arcy invented. We quite often recycle them - and I've never seen any problems. The thick ones are very easy to use because you can jam your thumb down on them without any danger of either cutting your thumb or losing your crystals. Alan (or someone) told me that the original ones were standard microscope (long) SLIDES cut into square sections by hand with a glass cutter. Hampton's are either round or square and some have beautiful flame-polished edges.
A chemist told me that once you siliconize glass you can never get the silane groups off again by any normal chemical means. He didn't see any need for eg rinsing with methanol and baking after treatment, which I had been doing. If recycling however the surface can become contaminated with grease and denatured protein. Either put them through a dishwasher (I've heard) or rub them hard with something soft. Thx, Patrick On Thu, 31 Jan 2019 at 17:14, Goldman, Adrian <adrian.gold...@helsinki.fi> wrote: > Yes, that was it! I just couldn’t remember the name. > > > On 31 Jan 2019, at 17:04, Mathews, Irimpan I. < > 00002add487f8799-dmarc-requ...@jiscmail.ac.uk> wrote: > > > > Long back we used Prosil solution (I think, 1% solution) for > siliconization. > > Mathews > > > > > > -----Original Message----- > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Goldman, Adrian > > Sent: Thursday, January 31, 2019 8:43 AM > > To: CCP4BB@JISCMAIL.AC.UK > > Subject: Re: [ccp4bb] Is there any alternative to siliconized glass > coverslips for crystallization? > > > > When I was a graduate student, about 150,000 years ago, we took regular > coverslips and doused them in ?silane to make siliconised ones. You then > let them sit in a rack to dry. It was a bit tedious but not horrendously > so. After a while, I stopped doing it altogether, because IMHO it didn’t > make a massive amount of difference to the behaviour of the solution on the > cover slip. It would bead up, or not, depending on what it was. > > > > So my advice would be: 1) siliconise yourself; 2) compare siliconised > versus non and decide if you can be bothered. > > > > Adrian Goldman > > > > > > > > On 31 Jan 2019, at 16:02, Holton, James M < > 0000270165b9f4cf-dmarc-requ...@jiscmail.ac.uk> wrote: > > > > plastic. > > > > Plastic cover slips are no good for UV or polarization, but they > are way better than glass if you happen to want to try in-situ diffraction. > (https://doi.org/10.1107/S0021889800001254) > > > > If you can't afford commercial ones, then you can always cut up > some inkjet transparency film sheets like McPherson did in the above > reference. Then after you've made a few hundred you can ask yourself how > much you'd be willing to pay somebody else to do it for you. There is no > wrong answer to that question, but it will determine which route you take. > > > > -James Holton > > MAD Scientist > > > > > > On 1/31/2019 12:17 AM, Rajnandani Kashyap wrote: > > > > > > Dear All > > I am a PhD student who requires lots of coverslips (!!) > for setting up hanging drop crystallization. The company sells it for a > huge amount. Also there is a wide monetary difference between a normal > siliconized coverslip and a 22mm siliconized circle coverslips. We tried to > search for an alternative companies but couldn't get any one who sells > coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm > glass diameter). Is there any alternative company (distribution in India) > from where we can buy them for a reasonable price? > > Thanks in advance for sparing your valuable time and > efforts. > > > > Regards > > Rajnandani Kashyap > > > > India > > > > > > ________________________________ > > > > > > To unsubscribe from the CCP4BB list, click the following > link: > > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > > > > > ________________________________ > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > > > > > ________________________________ > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > > > ######################################################################## > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
