Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

[Minmin Yu]

‎Agree. Nowadays I hardly do hanging drops and crystal clear tapes are good to seal the sitting drop plates too. I mainly use the plastic cover slips to set the cryo drops on for crystal harvesting. They can be put on any plates and the drops are good to be viewed under the microscope.  From: Frank Von Delft Sent: Friday, 1 February 2019 06:13 To: CCP4BB@JISCMAIL.AC.UK Reply To: Frank Von Delft Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Or do sitting drop, it's much easier all round. Frank Sent from tiny silly touch screen From: Minmin Yu <m...@mrc-lmb.cam.ac.uk> Sent: Thursday, 31 January 2019 21:42 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Yeah I used to use Fisher Scientific square plastic cover slips. Now I am using Sigma 22x22mm (Z36,590-4, 100 each Lot10B084279) smaller sized ones. These plastic ones are much more durable than the glass ones and are very easy to be handled. They work very well for both hanging drops or for sitting drops sealing with grease.  Minmin On 31 Jan 2019, at 21:14, Daniel M. Himmel, Ph. D. <danielmhim...@gmail.com> wrote: Fisher Scientific makes 22 inch square plastic cover slips, which are labeled "Fisherbrand Unbreakable Cover Slips". I and my colleagues have used them for over 15 years instead of siliconized glass coverslips. Just dip each one in ethanol to clean before use, dry with an air blower, and use it to set up  your drops.  They work like a charm.  They come 100 to a box.  Check the Fisher Scientific catalog. -Daniel On Thu, Jan 31, 2019 at 3:17 AM Rajnandani Kashyap <kashyap.rajnand...@gmail.com> wrote: Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price? Thanks in advance for sparing your valuable time and efforts. Regards Rajnandani Kashyap India To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1