Le Mercredi 27 Mars 2019 19:44 CET, Beatriz Gomes Guimaraes
<beatriz.guimar...@fiocruz.br> a écrit:
In four days from now, I (everyone) would have taken this as a Photoshop joke...
Quite amazing! Is it reproducible?
What is the protein? Tubulin ? ::))
Philippe Dumas
> Dear all,
>
>
> I would like to share with you a surprising pattern I found when examining
> some crystallization plates (attached figures).
>
>
> It is less obvious looking the photos, but apparently the "lines" are formed
> by precipitated protein and there are some "bubbles" with small drops inside.
> I wish they were microcrystals but I do not think this is the case.
>
> I was suprised by the symmetry !
>
>
> And it is not completely random because for the same condition the difference
> between the two drops are : protein alone ("hexagon") and protein + ligand
> ("rhombus")
>
>
> crystallization condition is:
>
> 0.01 M Cobalt(II) chloride hexahydrate
>
> 0.1 M Tris pH 8.5
>
> 20% w/v Polyvinylpyrrolidone K 15
>
>
> Have you seen anything similar before?
>
>
> Thank you for your comments!
>
> Beatriz
>
>
>
> --------------------------
> Beatriz Guimarães
> Laboratory of Structural Biology and Protein Engineering
> Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
> Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
> CIC 81350-010
> Curitiba - PR, Brasil
> Tel.:+55(41)3316-3225/2104-3438
>
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