I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing that the protein foams quite a bit, and that there were 6 (or four) more or less equal size bubbles that took up almost the whole drop when it was set up. Bubbles tend to pack quite efficiently (https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/), giving the base symmetry that stayed when the bubbles burst. Wondering if the lines are from the collapse of the thin protein/polymer skin covering the surface of the bubbles?
lovely and thought provoking images - thanks! Janet [https://physicsworld.com/wp-content/uploads/2013/05/bubbles.jpg]<https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/> Getting to the bottom of foamy physics – Physics World<https://physicsworld.com/a/getting-to-the-bottom-of-foamy-physics/> physicsworld.com Researchers in the US have created a new mathematical model to describe the complex evolution of foamy bubbles – something that has proved fiendishly difficult to model thanks to the hugely varying length and time scales involved. Their computed results closely match theoretical models as well as ... Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre (C3) CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email janet.new...@csiro.au ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Beatriz Gomes Guimaraes <beatriz.guimar...@fiocruz.br> Sent: Thursday, March 28, 2019 5:44 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Interesting pattern on a crystallization drop Dear all, I would like to share with you a surprising pattern I found when examining some crystallization plates (attached figures). It is less obvious looking the photos, but apparently the "lines" are formed by precipitated protein and there are some "bubbles" with small drops inside. I wish they were microcrystals but I do not think this is the case. I was suprised by the symmetry ! And it is not completely random because for the same condition the difference between the two drops are : protein alone ("hexagon") and protein + ligand ("rhombus") crystallization condition is: 0.01 M Cobalt(II) chloride hexahydrate 0.1 M Tris pH 8.5 20% w/v Polyvinylpyrrolidone K 15 Have you seen anything similar before? Thank you for your comments! Beatriz -------------------------- Beatriz Guimarães Laboratory of Structural Biology and Protein Engineering Instituto Carlos Chagas - ICC / FIOCRUZ Paraná Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C CIC 81350-010 Curitiba - PR, Brasil Tel.:+55(41)3316-3225/2104-3438 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
