Le Jeudi 28 Mars 2019 12:03 CET, jai mohan <00000cab66323371-dmarc-requ...@jiscmail.ac.uk> a écrit:
A friend of mine (Angel Piñeiro) just suggested to me the "Maragoni effect", which is likely active in the dancing droplets from Stanford and might explain the "explosion" into separate bubbles.... Indeed, one can see on Wikipedia a video illustrating how the contact of fluids with different surface tensions can have big effects... > May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his > work on Dancing Droplets, briefing the surface tension and evaporation ^ the > rule of two component fluids. # Since your precipitant contain PVP a shape > controlling agent > #https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html > > Best wishes > S.M.Jaimohan PhD > On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov > <sergei.strel...@kuleuven.be> wrote: > > #yiv3861306982 #yiv3861306982 > --P{margin-top:0;margin-bottom:0;}#yiv3861306982 > Artem (and Beatriz), > > > > > > Me bad, could have thought about that! I think you are right. > > > > > There were initially bubbles in each drop (7 in one case, 4 in the other). > > At some point the bubbles exploded (it was an instantaneous process, not just > shrinking). > > > > > Kind regards, > > Sergei > > > > > Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of > Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 > bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 > 32 Lab pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin > board <CCP4BB@JISCMAIL.AC.UK> on behalf of Artem Evdokimov > <artem.evdoki...@gmail.com> > Sent: Thursday, March 28, 2019 1:07 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat! > Looks like multiple adjacent bubbles that were initially touching but > eventually shrunk down to the central cores - the connectors are protein > filaments (skin on the bubbles) left over from when bubbles had contact > points. > Artem > On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) > <bevan.marsh...@csiro.au> wrote: > > > Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition > is found in both Index and JCSG screens as well as Classics II. > > > > > > Bevan Marshall > Staff Scientist | Collaborative Crystallisation Centre > Manufacturing > CSIRO > > E bevan.marsh...@csiro.aut +61 3 9662 7492 > 343-351 Royal Parade, Parkville, VIC 3052 > www.csiro.au|https://c3.csiro.au > CSIRO acknowledges the Traditional Owners of the lands that we live and work > on across Australia and pays its respect to Elders past and present. > > PLEASE NOTE > The information contained in this email may be confidential or privileged. > Any unauthorised use or disclosure is prohibited. If you have received this > email in error, please delete it immediately and notify the sender by return > email. Thank you. To the extent permitted by law, CSIRO does not represent, > warrant and/or guarantee that the integrity of this communication has been > maintained or that the communication is free of errors, virus, interception > or interference. > > Please consider the environment before printing this email. > > > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND > Pierre > Sent: Thursday, 28 March 2019 9:13 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop > > > > Dear Beatriz, > > > > Nice drops :-)) > > Could it be that there is a reaction going on in these drops ? > > The conditions are quite "exotic" with possibilities of coordination or > oxydoreduction (Co2+/Co3+) or polymerization... > > Do you have reductants with the protein buffer ? > > Is the protein an enzyme or a metalloprotein ? > > Just some ideas. > > > > Best wishes, > > Pierre > > > > De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes > Guimaraes [beatriz.guimar...@fiocruz.br] > Envoyé : mercredi 27 mars 2019 19:44 > À : CCP4BB@JISCMAIL.AC.UK > Objet : [ccp4bb] Interesting pattern on a crystallization drop > > Dear all, > > > > I would like to share with you a surprising pattern I found when examining > some crystallization plates (attached figures). > > > > It is less obvious looking the photos, but apparently the "lines" are formed > by precipitated protein and there are some "bubbles" with small drops > inside.I wish they were microcrystals but I do not think this is the case. > > I was suprised by the symmetry ! > > > > And it is not completely random because for the same condition the difference > between the two drops are : protein alone ("hexagon") and protein + ligand > ("rhombus") > > > > crystallization condition is: > > 0.01 M Cobalt(II) chloride hexahydrate > > 0.1 M Tris pH 8.5 > > 20% w/v Polyvinylpyrrolidone K 15 > > > > Have you seen anything similar before? > > > > Thank you for your comments! > > Beatriz > > > > > > -------------------------- > Beatriz Guimarães > Laboratory of Structural Biology and Protein Engineering > Instituto Carlos Chagas - ICC / FIOCRUZ Paraná > Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C > CIC 81350-010 > Curitiba - PR, Brasil > Tel.:+55(41)3316-3225/2104-3438 > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
