Hi everyone again Hmmm I think we have solved a structure in P1 space, to 2.5 A. However after refinement the Rfree stuck at 33%-35% with Rwork around 26%. The structure was solved by MR and current model seems to fit density well. In Refmac log I found that at the resolution corresponding to high R there may be a solvent/ice ring. Since imosflm should be able to exclude ice rings, I am not 100% sure whether it's the cause to high R. But if this is actually the case, is there a way I can exclude certain resolution bins during Refmac (and is it an appropriate way to do so?)
PS - the data is not affected by twining or pseudosymmetry as checked by Xtriage. Many thanks! Sam ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
