Look at the plots for Rfactor for your last cycle. Is there a blip at some resoution?
And also look at the plot of <Fobs> v <Fcalc> v resolution-- again are there blips? People have put a lot of effort in generating informative graphs so use them! You should see ice ring effects in the Wilson plot too.. Eleanor On Wed, 3 Apr 2019 at 19:41, Sam Tang <samtys0...@gmail.com> wrote: > Hi everyone again > > Hmmm I think we have solved a structure in P1 space, to 2.5 A. However > after refinement the Rfree stuck at 33%-35% with Rwork around 26%. The > structure was solved by MR and current model seems to fit density well. In > Refmac log I found that at the resolution corresponding to high R there may > be a solvent/ice ring. Since imosflm should be able to exclude ice rings, I > am not 100% sure whether it's the cause to high R. But if this is actually > the case, is there a way I can exclude certain resolution bins during > Refmac (and is it an appropriate way to do so?) > > PS - the data is not affected by twining or pseudosymmetry as checked by > Xtriage. > > Many thanks! > > Sam > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
