The key word for refmac is ANOM MAPONLY Are you using GUI2? Eleanor On Wed, 10 Jul 2019 at 09:32, Lumbini Yadav <lumbin...@gmail.com> wrote:
> I have soaked my crystals in sodium dithionite a reducing agent. I have > not done mass spec but sequence is confirmed > > On Tue, Jul 9, 2019 at 9:26 PM Bonsor, Daniel <dbon...@som.umaryland.edu> > wrote: > >> Have you mass-speced the protein before crystallization to make sure it >> wasn’t derivatized during expression and/or purification, or compared the >> mass spec of the crystals verses purified protein? Any fancy reagents or >> other reductants used during purification? >> >> What about S-Acetyl-cysteine (3-letter code: SCY). >> >> >> >> Best, >> >> >> >> Dan >> >> >> >> Daniel A Bonsor PhD. >> >> Sundberg Lab >> >> Institute of Human Virology >> >> University of Maryland, Baltimore >> >> 725 W Lombard Street N370 >> >> Baltimore >> >> Maryland >> >> MD 21201 >> >> Tel: (410) 706-7457 >> >> >> >> >> >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of >> *Lumbini Yadav >> *Sent:* Tuesday, July 09, 2019 5:22 AM >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* [ccp4bb] Fo-Fc density close to cysteine residue >> >> >> >> Dear all, >> >> >> >> We have found a huge Fo-Fc density close to cysteine residue (see >> attached image) in the structure with resolution of 1.2A. In the >> crystallization condition, we have PEG 3350, Potassium phosphate >> monobasic, glycerol and protein was in Tris and NaCl. Before freezing >> the crystals were soaked in mother liquor containing sodium dithionite. >> >> >> >> I have tried different modified cysteine (CSX, CSO, OCS, CME, CSS, SNC, CSD, >> CXM, SCH, CSU) from the library and also SO3, SO2 and peroxide. But in >> all the screenings we do see some part of Fo-Fc density unaddressed at 3 >> sigma. >> >> >> >> Does anyone have an idea about what this density could be? Covalent >> modification? >> >> >> >> Thanks. >> >> >> >> >> >> Kind regards, >> >> Lumbini >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
