I usually set a partial screen, maybe 24-48 conditions. If less than half the wells have precipitate, double protein concentration. If most have precipitate, maybe reduce protein or halve concentration of screen reagents. I usually start at 10 mg/mL or so. You can conveniently change protein conc. by manipulating protein/screen volume ratio.
__________________ Roger Rowlett On Wed, Jan 8, 2020, 11:16 AM Armando Albert <xalb...@iqfr.csic.es> wrote: > Dear all, > I was wondering how to guess the optimal protein concentration for the > initial crystallisation trials. Is there any trick or assay other than the > classic PCT from Hampton? > Armando > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
