I usually set a partial screen, maybe 24-48 conditions. If less than half
the wells have precipitate, double protein concentration. If most have
precipitate, maybe reduce protein or halve concentration of screen
reagents. I usually start at 10 mg/mL or so. You can conveniently change
protein conc. by manipulating protein/screen volume ratio.

__________________
Roger Rowlett

On Wed, Jan 8, 2020, 11:16 AM Armando Albert <xalb...@iqfr.csic.es> wrote:

> Dear all,
> I was wondering how to guess the optimal protein concentration for the
> initial crystallisation trials. Is there any trick or assay other than the
> classic PCT from Hampton?
> Armando
>
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